| Assay Method Information | |
| | Enzyme Assay |
| Description: | Aurora B (AurB): The 2×AURKB (Aurora B)/Ser/Thr 01 (ThermoFisher Scientific proprietary) mixture was prepared in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA. The final 10 μL Kinase Reaction consisted of 23 nM AURKB (Aurora B), 2 μM Ser/Thr 01 and 75 μM ATP (Km app measured as 81 μM ATP) in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA. After the 1 hour Kinase Reaction incubation, 5 μL of a 1:4096 dilution of Development Reagent was added. |
| Affinity data for this assay | |
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