| Assay Method Information | |
| | Inhibition Assay of HCoV-229E Viruses |
| Description: | HCoV-229E viral genome replication was examined by RT-PCR with specific primers against its open reading frame 1 (“ORF1”) and ORF nucleocapsid (“ORFN”). See Yang et al., Front. Pharmacol. 11, Article 606097 (2020). Fetus lung fibroblast MRC5 cells were inoculated with the HCoV-229E virus at a MOI of 1. The MRC5 cells thus infected were treated with one of Compounds 1-3. Untreated MRC5 cells were used as a control sample. RT-PCR analysis was performed. Compounds 1-3 each inhibited HCoV-229E viral genome replication and subgenomic viral RNA syntheses at a concentration between 10 nM and 300 nM. Cytopathic effects were studied and visualized after the infected cells were treated with one of Compounds 1-3. An end point assay by TCID50 was performed. Crystal violet was used to stain live cells to determine the EC50 of each compound. |
| Affinity data for this assay | |
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