Assay Method Information

Assay Name:  PI3K-alpha Enzyme Activity Assay
Description:  PI3K alpha-ADP Glo Assay was employed. In the determination of PI3K enzyme activity, the detection kit supplied by Promega (Promega, Cat #: V9101) was used to detect the inhibition effect of the compounds on the activity of PI3K enzyme. The adenosine diphosphate produced during the whole enzymic reactions was quantified.
Affinity data for this assay
 

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