Assay Method Information | |
| ChEMBL_1793118 (CHEMBL4265037) |
Description: | Inhibition of Escherichia coli PgaB expressed in Escherichia coli BL21 (DE3) cells transformed with pET28 plasmid coding for PgaB42-655 using compound pre-incubated with 1Eq DTT and using acetoxymethyl-4-methylumbelliferone as substrate measured at 2 min intervals over 10 mins in presence of ZnCl2 by fluorescence assay |
Affinity data for this assay | |
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