| Assay Method Information | |
| | AKT1 Inhibition Assay |
| Description: | The mixture of a S6-peptide, ATP, and test compound was added to the wells using a BioRAPTR FRD Workstation (Aurora Discovery). Reaction was started by addition of AKT1. After 55 min at RT the reaction was stopped by addition with a mixture of SreptavidinXL665 (Cisbio) and Eu3+ Cryptate labeled phospho S6 peptide antibody in quenching buffer. After 1 h incubation at RT the plate was measured on the Viewlux in HTRF mode. |
| Affinity data for this assay | |
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