| Assay Method Information | |
| | SARS-CoV-2 Plaque Assay 2 |
| Description: | The assay was performed in triplicate in 24-well tissue culture plates. Vero E6 cells were seeded in Dulbecco's modified Eagle's medium ( DMEM ) with 10% Fetal Bovine Serum ( FBS , Biological Industries, Kibbutz, Israel) and antibiotics one day before infection. SARS-CoV-2 viruses were added to the cell monolayer and allowed to sit for 1 hour at 37 C. After viruses were removed, the cell monolayer was washed once with PBS before covering with media containing agarose or methylcellulose for 5-7 days. The cells were fixed with 3.7% formaldehyde overnight followed by removal of overlay media. They were then stained with crystal violet to count the plaque-forming units ( PFU ). The percentage of inhibition was calculated as [1−(VD/VC)] 100%, where VD and VC refer to the virus titer in the presence and absence of a test compound, respectively. |
| Affinity data for this assay | |
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