| Assay Method Information | |
| | CYP Enzyme (Cytocrome P450) Inhibition Assay |
| Description: | 3.2. Inhibition of CYP2D6: Test group: The test compound of different concentration was added to the microplate, and Luciferin-ME EGE (3 μM), K3PO4 (100 mM) and CYP2D6 (5 nM) were added to each well, and pre-incubated at room temperature for 10 mM, followed by an addition of the NADPH regeneration system, and reacted at 37° C. for 30 min, an equal volume of detection buffer was added at last, and incubated at room temperature for 20 mM, and then chemiluminescence detection was performed. Negative control group: The experimental method was the same as that of the test group, but the test compound was not added. Blank control group: The experimental method was the same as that of the test group, but the test compound was not added, and CYP2D6 Membrance (5 nM) was used instead of CYP2D6. 3.3. Inhibition of CYP3A4: Test group: The test compound of different concentration was added to the microplate, Luciferin-IPA (3 μM), K3PO4 (100 mM) and CYP3A4 (2 nM) were added to each well, and pre-incubated at room temperature for 10 mM, followed by an addition of the NADPH regeneration system, and reacted at room temperature for 30 min, an equal volume of detection buffer was added at last, and incubated at room temperature for 20 mM, and then chemiluminescence detection was performed.Negative control group: The experimental method was the same as that of the test group, but the test compound was not added.Blank control group: The experimental method was the same as that of the test group, but the test compound was not added, and CYP3A4 Membrance (2 nM) was used instead of CYP3A4. |
| Affinity data for this assay | |
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