Assay Method Information | |
| ChEMBL_2014913 (CHEMBL4668491) |
Description: | Inhibition of N-terminal His6-tagged TEV cleavage site-fused human SHP2 catalytic domain (248 to 527 residues) expressed in Escherichia coli Rosetta(DE3) using 6,8-difluoro-4-methylum-belliferylphosphate as substrate preincubated for 1 hr followed by substrate addition by fluorescence method |
Affinity data for this assay | |
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