| Assay Method Information | |
| | D2 binding assay |
| Description: | 1 μL of a compound of the present disclosure and the reference compound (Table O) was transferred to an assay plate. 1 μL of droperidol was transferred to an assay plate according to the plate map for nonspecific binding (Low control: LC). 1 μL of DMSO was transferred to an assay plate according to the plate map for total binding (High control: HC). Following a plate map, 100 μL of the membrane stock solutions and radio ligand were added into the plate. The plates were sealed and shaken with 300 rpm.Unifilter-96 GF/C filter plates were soaked with 50 μL of 0.3% PEI per well for about 0.5 hours at room temperature. When the binding assays were complete, the reaction mixture was filtered through the GF/B filter plates using Perkin Elmer Filtermate Harvester, and each plate was washed 4 times with cold wash buffer. The filter plates were dried for 1 hour at 50° C. After drying, the bottom of the filter plate wells were sealed using Perkin Elmer Unifilter-96 backing seal tape. 50 μL of Perkin Elmer Microscint 20 cocktail was added. The top of the filter plates were sealed with Perkin Elmer TopSeal-A sealing film. |
| Affinity data for this assay | |
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