| Assay Method Information | |
| | A3 Adenosine Receptor Binding Assay |
| Description: | Aliquots of cell membranes (90 ug) were incubated at 25 °C for 180 min in 500 uL of binding buffer (50 mM Tris-HCl, 5 mM MgCl2, 1 mM EDTA, 2 units/mL ADA, pH 7.4) in the presence of 30 nm [3H]NECA and six different concentrations of the tested compounds. Non-specific binding was determined in the presence of 100 uM R-PIA. |
| Affinity data for this assay | |
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