| Assay Method Information | |
| | Luciferase-Based Assay |
| Description: | EphB4 kinase activity was similarly measured, using the luciferase-based technique described above. 28.9 mU/well EphB4 (Upstate) was reacted with 1 mg/ml poly(glu4tyr), 6 uM ATP and test reagents. The reaction was incubated for 60 minutes at 37 C and the residual ATP concentration was measured. |
| Affinity data for this assay | |
|---|---|
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