Assay Method Information | |
| ChEMBL_2336669 |
Description: | Inhibition of human recombinant full length METTL3/human N-terminal FLAG-tagged METTL14 expressed in SF9 cells assessed as methyltransferase residual activity using ssRNA 5'-rUrArC rArCrU rCrGrA rUrCrU rGrGrA rCrUrA rArArG rCrUrG rCrUrC -3' as substrate incubated for 20 mins followed by H-SAM addition measured after 2 hrs by radio isotope reaction system analysis |
Affinity data for this assay | |
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