| Assay Method Information | |
| | ChEMBL_1858218 (CHEMBL4358947) |
| Description: | Competitive inhibition of human N-terminal His6-tagged TEV protease site linked DHPS expressed in Escherichia coli BL21 (DE3) assessed as reduction in radiolabelled amino-butylidene incorporation in eIF5A using 100 uM of [3H]Spermidine trichloride as substrate after 90 to 120 mins in presence of eIF5A and 14 uM NAD+ by Topcount scintillation counting method |
| Affinity data for this assay | |
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