| Assay Method Information | |
| | ChEMBL_1816974 (CHEMBL4316548) |
| Description: | Inhibition of His-tagged JMJD6 (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as decrease in demethylase activity by measuring release of formaldehyde using core histone as substrate preincubated with enzyme for 10 mins followed by incubation with substrate for 1 hr measured following 2 hrs incubation with ammonium acetate/acetoacetanilide by fluorescence based analysis |
| Affinity data for this assay | |
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