| Assay Method Information | |
| | MMP Inhibition Assay |
| Description: | This assay was performed as outlined in the Anaspec MMP assay kit. The fluorescence peptide cleavage assay was performed in a 96-well plate in which each reaction mixture contained 5-FAM / QXL520 and activated MMP-2 or MMP-9 in assay buffer, and the screening compounds. Kinetics of the peptide cleavage was examined every 5 min for 30 min by using a fluorescence plate reader at excitation and emission wavelengths of 485 and 535 nm, respectively, and percent inhibition values were obtained. |
| Affinity data for this assay | |
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