| Assay Method Information | |
| | Norepinephrine Transporter Binding Assay |
| Description: | Human Norepinephrine Transporter (NET) Binding (Antagonist Radioligand) was determined at Panlabs Cerep (assay #204410). Human norepinephrine transporters expressed in dog kidney MDCK cells were used in modified Tris-HCl buffer pH 7.4. A 40 μg‡ aliquot was incubated with 0.2 nM [125I]RTI-55 for 3 hours at 4° C. Non-specific binding was estimated in the presence of 10 μml desipramine. Membranes were filtered and washed, and the filters were then counted to determine [125I]RTI-55 specifically bound. Compounds were assayed using an 8-point titration, with a starting concentration of 10 μM and one-half log serial dilutions. ‡Note: Membrane protein may change from lot to lot, the concentration used is adjusted if necessary. IC50 values were determined by a non-linear, least squares regression analysis using MathIQ™ (IDBusiness Solutions Ltd., UK). |
| Affinity data for this assay | |
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