| Assay Method Information | |
| | hHDAC2 Inhibition Protocol |
| Description: | The buffer assay used in the inhibition hHDAC2 assay is: Hepes 50 mM, KCl 100 mM, Tween 20 0.001%, BSA 0.01%; pH=7.4. Study compound and HDAC2 (BPS Bioscience 50002) enzyme 12 nM was added in a 384 well Microplate (Geriner 784209) and incubated during 3 hours at RT. Later Acetylated Peptide A (Perkin Elmer CLS960006) 1 μM was added and incubated during 1 hour at RT. Finally, LBH589 (Reaction Biology Corp EP1009B) 1.4 μM was added to stop the reaction. The reaction was measured in a Caliper EzReader LabChip 3000 (Caliper, Hopkinton, MA) reader. hHDAC2, LBH-589 (Reaction Biology Corp EP1009B) IC50<3 nM, Gale et al, Application note Perkin Elmer. |
| Affinity data for this assay | |
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