| Assay Method Information | |
| | hERG Inhibition Assay |
| Description: | A manual patch-clamp technology is used to evaluate whether the test compound has a potential inhibitory effect on a voltage-gated potassium ion channel hERG. In this experiment, the effects of 5 concentrations of compound (2 parallel samples per concentration) on the current of the hERG channel are detected, a dose-response curve of the compound is obtained and IC50 is calculated. First, the hERG current measured in the extracellular fluid containing 0.1% DMSO is used as a baseline for detection. After the hERG current remains stable for at least 5 min, the solution containing the test compound is perfused sequentially around the cells from low to high concentration. It is necessary to wait about 5 min after each perfusion to allow the compound to act fully on the cells and record the hERG current synchronously. After the recorded current tends to stabilize, the last five hERG current values are recorded, and averaged as a final current value at a specific concentration. After testing the compound, 150 nM Dofetilide is added to the same cell to completely inhibit its current as a positive control for this cell. At the same time, the positive compound Dofetilide is simultaneously detected with the same patch-clamp system before and after the end of a test drug experiment to ensure the reliability and sensitivity of the entire detection system. |
| Affinity data for this assay | |
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