| Assay Method Information | |
| | Electrophysiological Assay |
| Description: | CHO (Chinese Hamster Ovary) cells stably expressing hERG potassium channels were taken, and hERG potassium channel currents were recorded at room temperature by means of a whole-cell patch-clamp technique. A glass microelectrode was formed by pulling a glass electrode blank (BF150-86-10, Sutter) with a glass microelectrode puller. The tip resistance was about 2-5 MΩ after perfusion of a pippette solution. The glass microelectrode could be connected to a patch clamp amplifier by inserting the glass microelectrode into an amplifier probe. Clamping voltages and data recording were controlled and recorded by a computer with pClamp 10 software, with a sampling frequency of 10 kHz and a filtering frequency of 2 kHz. After the whole-cell recording was obtained, the cells were clamped at −80 mV, and the step voltage evoking hERG potassium current (I hERG) was changed from −80 mV to +20 mV by giving a 2 s depolarization voltage and then repolarized to −50 mV for 1 s, and returned to −80 mV. Such voltage stimulation was given every 10 s, and the administration process was started after it was determined that the hERG potassium currents were stable (1 min). The compound at each test concentration was given at least 1 min, and at least 2 cells (n≥2) were tested for each concentration. |
| Affinity data for this assay | |
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