| Assay Method Information | |
| | LRRK2 and LRRK2 G2019S Kinase Assay |
| Description: | The LRRK2 and LRRK2 G2019S kinase assays were performed using the AdaptaTM technology in ThermoFisher Scientific. This experiment was carried out according to the supplier s protocol. Briefly, assay conditions were as follows. The mixture of substrate (LRRKtide) and each kinase was prepared in 50 mM Tris pH 8.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA, 0.02% NaN3. Assays were performed in the presence of 70 M ATP and 100 M ATP (KmATP) in LRRK2 and LRRK2 G2019S, respectively. The reaction was progressed at room temperature for 1 hour. Upon completion of kinase reaction, 5 L of Detection Mix was added and after 60 min incubation time, the emission ratio of 665/615 nm was calculated. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |