| Assay Method Information | |
| | NMR Chemical Shift Titration Assay |
| Description: | NMR chemical shift perturbations of PTP1B resonances due to binding of CHA and CGA were monitored by acquiring a series of 1H−15N TROSY spectra of 15N- and 2H-labeled PTP1B upon addition of increasing concentrations of CHA and CGA. For CHA, spectra were recorded at 0.00, 0.20, 0.40, 1.00, 3.00, and 5.00 mM CHA from a 150 mM CHA stock into 0.38 mM [15N,2H]PTP1B (550 μL starting volume). For CGA, spectra were recorded at 0.00, 0.11, 0.21, 0.52, 1.04, 5.20, and 8.32 mM CGA using a 156 mM CGA stock titrated into 0.38 mM [15N,2H]PTP1B (550 μL starting volume). |
| Affinity data for this assay | |
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