| Assay Method Information | |
| | Fluorescence Binding Assays |
| Description: | Varying amounts of enzyme were diluted in PBS supplemented with 0.5 g/L pluronic F127 and incubated with 500 nM ML349-FL for 30 minutes at room temperature. The fluorescent polarization from each well was measured using a Tecan F500 plate reader using 485/20 nm excitation and 535/25 nm emission polarization filters. Each concentration was measured in 2 replicates. |
| Affinity data for this assay | |
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