| Assay Method Information | |
| | A2A Adenosine Receptor Binding Assay |
| Description: | Aliquots of cell membranes (30 ug) were incubated at 25 °C for 90 min in 500 uL of binding buffer (50 mM Tris-HCl, 2 mm MgCl2, 2 units/mL ADA, pH 7.4) in the presence of 30 nM of [3H]NECA and six different concentrations of the tested compounds. Non-specific binding was determined in the presence of 100 uM R-PIA. The dissociation constant (Kd) of [3H]NECA in human A2A AR CHO cell membranes was 30 nM. |
| Affinity data for this assay | |
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