| Assay Method Information | |
| | ABTS Free-Radical Cation Scavenging Assay |
| Description: | The ABTS free radical cation scavenging ability of the synthe- sized compounds was determined according to the procedure described earlier [Free Radical Biol. Med. 26(9-10):1231-1237]. ABTS was dissolved in distilled water (7x10^3 M) and potassium persulphate (2.45x10^3 M) was added. This reaction mixture was left overnight (12-16 h) in the dark, at room temperature. Various concentrations of test substances (1000 µg/mL 500 µg/mL, 250 µg/mL, 100 µg/mL, 50 µg/mL, 25 µg/mL, 10 µg/mL concentrations) were incubated with the ABTS+P solution for 30min. The absorbance was measured at 734 nm, and the % inhibition was calculated using the formula described in Eq. (1) and the IC50 was calculated. Ascorbic acid was used as the positive control. |
| Affinity data for this assay | |
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