| Assay Method Information | |
| | Butyrylcholinesterase Assay |
| Description: | The butyrylcholinesterase (BChE) inhibition activity was determined according to the Ellman's method with minor modifications. Total volume of the reaction mixture was 100 µL containing 60 µL, Na2HPO4 buffer, 50 mM and pH 7.7. Ten µL test compound 0.5 mM well-1, followed by the addition of 10 µL (0.5 unit well-1)BChE. The contents were mixed and pre-read at 405 nm and then pre-incubated for 10 min at 37 °C. The reaction was initiated by the addition of 10 µL of 0.5 mM well-1 substrate (butyrylthiocholine bromide) followed by the addition of 10 µL DTNB, 0.5 mM well-1. After 30 min of incubation at 37 °C, absorbance was measured at 405 nm. Synergy HT (BioTek, USA) 96-well plate reader was used in all experiments. All experiments were carried out with their respective controls in triplicate. |
| Affinity data for this assay | |
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