| Assay Method Information | |
| | AChE and BChE Assay |
| Description: | The AChE inhibition assay was performed according to the Ellman's method. Total volume of 100 µL reaction mixture contained 60 µL 50 mM Na2HPO4 buffer, pH 7.7. Ten µL test compound (0.5 mM well#1) was added, followed by the addition of10 µL (0.005 unit well#1 AChE, 0.5 unit well#1 BChE, Sigma Inc.) enzyme. The contents were mixed, pre-incubated for 10 min at 37 °C and pre-read at 405 nm. The reaction was initiated by the addition of 10 µL of 0.5 mM well#1 substrate (acetylthiocholine iodide or butyrylthiocholine chloride, Sigma Inc.), followed bythe addition of 10 µL DTNB from Sigma Inc. (0.5 mM well#1). After 15 min of incubation at 37 °C, absorbance was measured using 96-well plate reader Synergy HT, Biotek, USA. All experiments were carried out with their respective controls in triplicate. Eserine (0.5 mM well#1) was used as a positive control. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |