| Assay Method Information | |
| | HDAC8 Inhibition Assay (kinetic method) |
| Description: | To determine the dissociation rate of the inhibitor, 1 µM HDAC8 and 20 µM inhibitor (syringe I) were mixed with 100 µM c-SAHA (syringe II) via the stopped-flow system. The fluorescence was measured at 325 nm with a 395 nm cutoff filter. |
| Affinity data for this assay | |
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