| Assay Method Information | |
| | ChEMBL_1982607 (CHEMBL4615869) |
| Description: | Inhibition of wild type HIV1 subtype B NL4-3 6xHis-tagged integrase expressed in Escherichia coli BL21 (DE3) assessed as inhibition of 3'-processing and strand-transfer reactions using DNA as substrate preincubated with enzyme for 1 hr followed by addition of recombinant 6xHis-tagged LEDGF/p75 expressed in Escherichia coli BL21 (DE3), DNA donor substrate and DNA acceptor substrate and further incubated for 90 mins by HTRF assay |
| Affinity data for this assay | |
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