Assay Method Information

Assay Name:  ChEMBL_1793117 (CHEMBL4265036)
Description:  Inhibition of Escherichia coli PgaB expressed in Escherichia coli BL21 (DE3) cells transformed with pET28 plasmid coding for PgaB42-655 using compound pre-incubated with 1Eq DTT and using acetoxymethyl-4-methylumbelliferone as substrate measured at 2 min intervals over 10 mins in presence of CoCl2 by fluorescence assay
Affinity data for this assay
 

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