| Assay Method Information | |
| | ChEMBL_1740513 (CHEMBL4156263) |
| Description: | Inhibition of recombinant human N-terminal His6-tagged SIRT1 (241 to 512 residues) expressed in Escherichia coli Transetta(DE3) using AcIQF as substrate preincubated for 30 mins followed by substrate and NAD+ addition measured after 2 hrs by fluorescence assay |
| Affinity data for this assay | |
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