Assay Method Information

Assay Name:  In Vitro Kinase Assay (LanthaScreen Kinase Assay)
Description:  Compounds were tested for their ability to inhibit JNK1 phosphorylation of ATF2 in the Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) based LanthaScreen kinase assay (Invitrogen). The signal was measured at 520/495 nm emission ratio on a fluorescence plate reader (Victor 2, Perkin-Elmer).
Affinity data for this assay
 

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