| Assay Method Information | |
| | Adenosine A1 Receptor Binding Assay |
| Description: | The displacement of [3H]DPCPX binding from the A1AR was determined using DDT1 MF-2 cell membranes. Nonspecific binding was determined in parallel assays that contained 1 uM alprenolol. The radioactivity was determined in a gamma counter. All assays were performed in triplicate. The concentration of compounds that inhibited radioligand binding by 50% (IC50) was obtained by nonlinear regression analysis using GraphPad Prism. The dissociation constant (Ki) was obtained from IC50 value by using the conversion described by Cheng and Prusoff. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |