| Assay Method Information | |
| | CDK5/p35 Assay |
| Description: | CDK5/p35 activity was determined in DELFIA format using a biotinylated Histone H1 peptide. The phosphorylated peptide was quantified using rabbit phospho-cdk1 substrate polyclonal antibody and DELFIA europium-labelled anti-rabbit IgG secondary antibody using time-resolved fluorescence (excitation@335nm, emission@620nm). IC50 values were calculated from replicate curves, using GraphPad Prism software. |
| Affinity data for this assay | |
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