| Assay Method Information | |
| | H3R Radioligand Binding Assay |
| Description: | Ligand displacement assays were performed on CHO cells membranes expressing hH3R. Retained radioactivity was determined by liquid scintillation counting. Nonspecific binding was determined in the presence of 1 uM thioperamide. The Ki values were calculated based on an experimentally determined appropriate Kd value according to Cheng and Prusoff. |
| Affinity data for this assay | |
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