| Assay Method Information | |
| | In vitro BuChE Inhibition Assay |
| Description: | Inhibition of BuChE activity was determined by the spectroscopic method of Ellman using butyrylthiocholine iodide as substrate, in 96-well microtiter plates. The reaction was initiated by adding butyrylthiocholine iodide to each well containing reaction mixtures, and was followed by monitoring the appearance of the thiolate dianion produced by reduction of DTNB at 412 nm for 120 s in a Molecular Devices Spectra Max 384 Plus plate reader. Each inhibitor was evaluated at several concentrations in the range of 10-9 to 2 x10-5 M. Percentage inhibition was calculated relative to a control sample (DMSO), and IC50 values displayed represent the mean +/- standard deviation for triplicate assays. |
| Affinity data for this assay | |
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