Assay Method Information

Assay Name:  Cathepsin D Enzymatic Assay
Description:  Cathepsin D activity was measured at pH 4 using a FRET peptide substrate. Compounds were preincubated with recombinant human liver cathepsin D for 20 min before adding substrate. Cleavage of substrate was monitored on a Flexstation 384 (Ex 320 nm/Em 400 nm) for 20 min.
Affinity data for this assay
 

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