Assay Method Information | |
| Cathepsin D Enzymatic Assay |
Description: | Cathepsin D activity was measured at pH 4 using a FRET peptide substrate. Compounds were preincubated with recombinant human liver cathepsin D for 20 min before adding substrate. Cleavage of substrate was monitored on a Flexstation 384 (Ex 320 nm/Em 400 nm) for 20 min. |
Affinity data for this assay | |
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