| Assay Method Information | |
| | FLT3 Inhibition Assay |
| Description: | The compounds were tested for their ability to inhibit the phosphorylation of poly(Glu:Tyr) by purified recombinant human FLT3. The extent of phosphorylation was quantitated by a fluorescent polarization competition immunoassay (Ex: 485 nm, Em:530 nm) using an anti-phosphotyrosine antibody and a phosphotyrosine tracer (Green Tyrosine Kinase Assay Kit, PanVera P2837). |
| Affinity data for this assay | |
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