| Assay Method Information | |
| | 5-HT2C Receptor Binding Inhibition Test |
| Description: | In advance, 0.5 μL of the compound solution dissolved in DMSO is dispensed into a microplate, and the cell membrane and the hot ligand are diluted with Assay buffer, respectively. Then, the Assay buffer containing the diluted cell membrane is dispensed into a microplate at 50 μL/well. Then, the radioactive ligand solution is dispensed into a microplate at 50 μL/well, and the plate is sealed. Then, it is allowed to stand at 37° C. for 2 hours. During this period, 50 mmol/L Tris-HCl (pH 7.4) is dispensed into a GF/B UniFilter plate at 50 μL/well and allowed to stand at 4° C. for 1 hour or longer. After that, filtration is performed with Cell harvester (PerkinElmer). After the GF/B UniFilter plate is dried at room temperature, MicroScinti 20 is dispensed into the GF/B UniFilter plate at 50 μL/well, and the plate is sealed. The GF/B UniFilter plate is allowed to stand overnight at room temperature. The radioactivity of [3H]-Mesulergine bound to the 5-HT2C receptor is measured using Microbeta2 (PerkinElmer) at a measurement time of 1 min/well. |
| Affinity data for this assay | |
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