| Assay Method Information | |
| | CPE Suppression Effect Confirmation Test |
| Description: | MDCK cells were seeded in a 96-well cell culture plate, and cultured till cell attachment for later use. The drug was serially 3 times diluted from 2 times the highest test concentration to 8 gradients. The drug was added to the cells and cultured in a CO2 incubator at 37° C. After 48 hours of dosing and incubation, the cytopathic effect (CPE) caused by the drug was observed under a microscope. Then alamarBlue® medium was added to detect the survival rate of the cells. The toxicity of the drug to cells is inversely proportional to the activity of the cells and is expressed as the activity of the cells. |
| Affinity data for this assay | |
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