| Assay Method Information | |
| | CB1 and CB2 Binding Inhibition by Compounds Purified from Milicia excelsa (African Teak) |
| Description: | The organic extract (8 g) from the stem barks of Milicia excelsa, obtained using the methods described in Example 1, was divided and loaded separately onto two pre-packed flash columns (120 g silica, particle size 32-60 μm, 4 cm×19 cm), then the column was eluted with the gradient as described in Example 5. A Diels-Alder adduct of a chalcone and prenylphenyl moiety was isolated from one of the active fractions and identified as Sanggenon C/D/. |
| Affinity data for this assay | |
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