| Assay Method Information | |
| | TDO2 Enzymatic Inhibition Assay |
| Description: | A Standard reaction mixture (200 uL/well) containing 50 mM potassium phosphate buffer (pH 6.5), 20mM ascorbic acid (neutralized with NaOH), 200 ug/ml catalase, 10uM methylene blue, 12.5 ug/mL recombinant human IDO1 and 500 uM L-Tryptophan was added to test compound dissolved in DMSO at a determined concentration. The mixture was incubated for 1 hour at 37C and the reaction was stopped by adding 40uL/well of 30% (w/v) trichloroacetic acid. After heating at 65C for 15 min, 125uL was transferred into a well of a 96-well microplate and mixed with 125uL of 2% (w/v) p-dimethylaminobenzaldhyde in acetic acid. |
| Affinity data for this assay | |
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