Assay Method Information

Assay Name:  ChEMBL_1561039 (CHEMBL3776335)
Description:  Inhibition of KDM6B (unknown origin) expressed in Escherichia coli using H3 (21 to 44 residues) K27Me3-GGK-Biotin JMJD3 Cognate Ligand/10 uM alpha-ketoglutarate as substrate/cofactor preincubated for 5 mins followed by substrate addition measured after 5 mins by alphascreen assay
Affinity data for this assay
 

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