| Assay Method Information | |
| | HDAC10 Inhibition Protocol |
| Description: | The buffer assay used in the inhibition hHDAC10 assay is: Hepes 50 mM, KCl 100 mM, Tween 20 0.001%, BSA 0.01%; pH=7.4. Study compound and hHDAC10 (BPS Bioscience 50010) enzyme 10 nM was added in a 384 well Microplate (Geriner 784209) and incubated during 60 minutes at RT. Later Acetylated Peptide A (Perkin Elmer CLS960006) 6 μM was added and incubated during 24 hours at RT. Finally, LBH589 (Reaction Biology Corp EP1009B) 1.4 μM was added to stop the reaction. The reaction was measured in a Caliper EzReader LabChip 3000 (Caliper, Hopkinton, MA) reader. |
| Affinity data for this assay | |
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