| Assay Method Information | |
| | Activity Assay |
| Description: | The activity assay for the compound of each example was carried out in this experimental example, and the detailed test method was as follows:1. The test compound was formulated as 10 μmol/L solution in dimethylsulfoxide;2. Starting from 10 μmol/L, by 3-fold serial dilution, the inhibitory activity of each compound against the enzyme was tested in singlet state at 10 doses;3. IC50 values of test compounds were finally obtained. |
| Affinity data for this assay | |
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