| Assay Method Information | |
| | Binding Assay |
| Description: | S1P1 membrane is prepared from CHO-K1 Gαqi5 cells expression full-length human S1P1. Scintillation proximity assay (SPA) is performed by incubating membranes, GTPγ35S, and compounds at various concentrations for 60 minutes. Wheat germ agglutinin-coated SPA beads are added and incubated for 60 minutes before centrifugation and scintillation counting. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |