| Assay Method Information | |
| | Inhibition Activity (ELISA Method) Assay |
| Description: | In order to evaluate the activity of the compounds of the present invention as a BTK inhibitor, commercially available BTK (Promega) was used for this experiment. Specifically, an enzymatic reaction was conducted by mixing 0.4 nM of BTK enzyme, 40 μM of biotin-S1 substrate peptide and 50 μM of ATP in a reaction buffer (15 mM Tris-HCl (pH 7.5), 20 mM MgCl2, 2 mM MnCl2, 2 mM DTT, 0.1 mg/ml BSA). The mixture was treated with the test compounds at predetermined concentrations and allowed to react for 20 minutes at 30° C. Upon completion of the reaction, the activities of the test compounds were measured by ELISA method. The absorbance value of an untreated sample was used as a control (100% control) |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |