| Assay Method Information | |
| | Fluorescence Polarization Displacement |
| Description: | Twelve compounds from Schemes 1 and 2 were screened using fluorescence polarization, for their ability to bind ERα (Table 1). Only six compounds showed any significant affinity for the receptor at concentrations as high as 1 μM. These compounds include five of the six steroid-core compounds 2, 4, 7, 11, and 13 and one bicyclic compound 18. Of the remaining six compounds which did not bind to ERα, one has the steroid core while the others contain the linked ring cores containing a flanking hydroxyl group a structure whose hydrophobic interior and hydrophilic exterior resembles that of estrogen itself. |
| Affinity data for this assay | |
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