| Assay Method Information | |
| | enzymatic assay |
| Description: | Recombinant SARS-CoV-2 Mpro was expressed and purified from Escherichia coli (E. coli) (18, 25). A fluorescently labeled substrate, MCA-AVLQ SGFR-Lys (Dnp)-Lys-NH2, derived from the N-terminal auto-cleavage sequence from the viral protease was designed and synthesized for the enzymatic assay. |
| Affinity data for this assay | |
|---|---|
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