| Assay Method Information | |
| | Fluorescence Resonance Energy Transfer (FRET)-Based Assay |
| Description: | The assay was performed with plasmepsin-II/cathepsin D (1.2 nM) and substrate (malaria FRET-1; 1.0 μM) in 0.1 M sodium acetate buffer pH 5, containing 10% glycerol and 0.01% Tween 20. The hydrazide and hydrazine compounds dissolved in DMSO were added in the reaction mixture before the addition of substrate. The assays were performed with 5% final concentration of DMSO. The enzyme inhibition experiments were performed (in triplicates) in 96-well plate format and readings were obtained on a Perkin Elmer LS55 fluorescence spectrometer, Waltham, Massachusetts, with an excitation and emission wavelengths of 336 and 490 nm respectively. |
| Affinity data for this assay | |
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