| Assay Method Information | |
| | Biological Assay |
| Description: | The synthesized compounds were evaluated for their ability to inhibit DHFR from pc, tg, ma, and rl using a continuous spectrophotometric assay measuring oxidation at 340 nM of NADPH at 37°C under conditions of saturating substrate and cofactor as previously described [Broughton et al., Antimicrob. Agents Chemother., 35:1348-55; Chio et al., Antimicrob. Agents Chemother., 37:1914-23]. |
| Affinity data for this assay | |
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